Device

Part:BBa_K2936013:Design

Designed by: Yu Weng   Group: iGEM19_ZJUT-China   (2019-10-09)


Formaldehyde degradation system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 904
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1384
    Illegal NotI site found at 1745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 904
    Illegal BglII site found at 1265
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 904
    Illegal PstI site found at 1384
    Illegal NgoMIV site found at 853
    Illegal NgoMIV site found at 1049
    Illegal NgoMIV site found at 1402
    Illegal NgoMIV site found at 1662
    Illegal NgoMIV site found at 2138
    Illegal NgoMIV site found at 2249
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

An RBS sequence can be inserted between flhA and fghA genes to speed up translation.


Source

The main components from Paracoccus denitrificans.

References